KMID : 1134819970260061228
|
|
Journal of the Korean Society of Food Science and Nutrition 1997 Volume.26 No. 6 p.1228 ~ p.1236
|
|
Isolation of Bacteriocin - producing Lactic Acid Bacteria from Human Intestines and the Characteristics of their Bacteriocins
|
|
Maeng Kil-Jae
Kim Jeong-Sang Ji Geun-Eog Kim Jeong-Hwan
|
|
Abstract
|
|
|
Lactobacillus strains were isolated from volunteer¡¯s feces, including from newly-born infants and adults in their 20¡¯s, by using differential MRS-BPB plates. Total 56 presumptive Lactobacillus strains were isolated and the bacteriocin productions by the isolates were examined by agar diffusion method. Six bacteriocin-producing strains were confirmed. Among them, two isolates, HU-1 and H22-3, showed the most outstanding antimicrobial activities, which were not affected by pH adjustments or catalase treatments of culture. HU-1 was originated from a two-years old boy and H22-3 was originated from a newly-born infant. HU-1 and H22-3 had the same morphology(short rod) when examined by scanning electron microscope, and the same biochemical traits including growth temperature range, salt tolerance and sugar-fermenting abilities. But the growth-inhibition spectrum and plasmid profiles of HU-1 and H22-3 were different. Both strains inhibited the growth of various Gram (+) microorganisms including Listeria monocytogenes, Micrococcus luteus, and Staphylococcus aureus in addition to many species of lactic acid bacteria, indicating the production of broad-spectrum bacteriocins. Bacteriocins produced by HU-1 and H22-3 were stable up to 90¡É, 15 min heat treatments. Their activities were not affected by pepsin or trypsin treatments but destroyed by proteinaseK or pronase treatments.
|
|
KEYWORD
|
|
bacteriocins, human intestines, lactic acid bacteria
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|